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| Zhang,Xiaochao; Yang,Renhua; Long,Jiang; He,Bo; Zhang,Yue; Fu,Ting; Shen,Zhiqiang; Chen,Peng. |
| In this study, the effects of geraniin on osteoprotegerin/receptor activator of nuclear factor-κB ligand(OPG/RANKL) in regulating the proliferation of osteoblasts and suppression of osteoclast-like cells (OLC) in OLC-osteoblast co-cultured system in vitro were investigated. Osteoblasts were cultured and identified with alkaline phosphatase (ALP), gomori stain, and mineralized nodule stain. OLCs were isolated from long bones of Sprague-Dawley (SD) rats and identified with tartrate-resistant acid phosphatase(TRAP) stain. Methyl thiazolyl tetrazolium assay was used to examine the proliferation of osteoblasts, and immunocytochemistry and in situ hybridization to analyze the expression OPG/RANKL in osteoblasts co-cultured with osteoclasts under the action of... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Geraniin/effect; Osteoblasts; Osteoprotegerin; Receptor activator of nuclear factor -κ B ligand.. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502018000300618 |
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| Zhang,L.; Ding,Y.; Rao,G.Z.; Miao,D.. |
| The effects of interleukin-10 (IL-10) and glucose on mRNA and protein expression of osteoprotegerin (OPG), and its ligand, receptor activator of nuclear factor-κB ligand (RANKL), were investigated in human periodontal ligament fibroblasts (HPDLFs). Primary HPDLFs were treated with different concentrations of IL-10 (0, 1, 10, 25, 50, and 100 ng/mL) or glucose (0, 5.5, 10, 20, 30, and 40 mmol/L). Changes in mRNA and protein expression were examined using the reverse-transcription polymerase chain reaction (RT-PCR) and Western blot analysis, respectively. After IL-10 treatment, mRNA and protein levels of OPG were increased, while mRNA and protein levels of RANKL were decreased (P<0.05), both in a concentration-dependent manner. Glucose stimulation had the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Human periodontal ligament fibroblasts; Interleukin-10; Glucose; Osteoprotegerin; Receptor activator of nuclear factor-κB ligand. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016000400605 |
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| Nascimento,M.M.; Hayashi,S.Y.; Riella,M.C.; Lindholm,B.. |
| Osteoprotegerin (OPG) regulates bone mass by inhibiting osteoclast differentiation and activation, and plays a role in vascular calcification. We evaluated the relationship between osteoprotegerin levels and inflammatory markers, atherosclerosis, and mortality in patients with stages 3-5 chronic kidney disease. A total of 145 subjects (median age 61 years, 61% men; 36 patients on hemodialysis, 55 patients on peritoneal dialysis, and 54 patients with stages 3-5 chronic kidney disease) were studied. Clinical characteristics, markers of mineral metabolism (including fibroblast growth factor-23 [FGF-23]) and inflammation (high-sensitivity C-reactive protein [hsCRP] and interleukin-6 [IL-6]), and the intima-media thickness (IMT) in the common carotid arteries... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Atherosclerosis; Chronic kidney disease; Inflammation; Mortality; Osteoprotegerin. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014001100995 |
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| Benslimane-ahmim, Z.; Heymann, D.; Dizier, B.; Lokajczyk, A.; Brion, R.; Laurendeau, I.; Bieche, I.; Smadja, D. M.; Galy-fauroux, I.; Colliec-jouault, Sylvia; Fischer, Anne-marie; Boisson-vidal, Catherine. |
| Background: Osteoprotegerin (OPG), a soluble receptor of the tumour necrosis factor family, and its ligand, the receptor activator of nuclear factor-kappa B ligand (RANKL), are emerging as important regulators of vascular pathophysiology. Objectives: We evaluated their effects on vasculogenesis induced by endothelial colony-forming cells (ECFC) and on neovessel formation in vivo. Methods: Effects of OPG and RANKL on in vitro angiogenesis were evaluated after ECFC incubation with OPG or RANKL (0-50 ng mL(-1)). Effects on microvessel formation were evaluated with an in vivo murin Matrigel plug assay. Vascularization was evaluated by measuring plug hemoglobin and vascular endothelial growth factor (VEGF)-R2 content 14 days after implantation. Results: We... |
| Tipo: Text |
Palavras-chave: Endothelial colony-forming cells; Osteoprotegerin; Receptor activator of nuclear factor-kappa B ligand; Vasculogenesis. |
| Ano: 2011 |
URL: http://archimer.ifremer.fr/doc/00033/14462/11863.pdf |
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